My research is interdisciplinary and focused on developing new types of fluorescence microscopes that could advance the work of other researchers and medical personnel in a wide variety of fields. My research interests include multiphoton microscopy (MPM), fluorescence lifetime imaging microscopy (FLIM), super-resolution microscopy, high-speed imaging, deep tissue imaging, adaptive optics, computational imaging, and deep learning. In general, I design and build novel fluorescence microscopes with improved imaging speed, resolution, depth, and functionality over conventional microscopes.
- Super-Sensitivity MPM-FLIM
- Saturation-Compensated FLIM
- Phase-Multiplexing FLIM
- Super-Resolution: Stepwise Optical Saturation (SOS)
- Super-Resolution: Deconvolution Stepwise Optical Saturation (DeSOS)
- Super-Resolution FLIM: Generalized Stepwise Optical Saturation (GSOS)
- Sensorless Adaptive Optics
- Image Denoising by Deep Learning
- FLIM Phasor Plots
- Automatic Image Segmentation by K-Means Clustering
Here is an example of the images I acquired using a custom-built phase-multiplexing MPM-FLIM:
Yide Zhang, Ian H. Guldner, Evan L. Nichols, David Benirschke, Cody J. Smith, Siyuan Zhang, and Scott S. Howard, “Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics”, SPIE Photonics West 2019, San Francisco, California USA, Feb. 2019.
Here is a comparison between conventional and DeSOS microscopy images:
Yide Zhang, Evan L. Nichols, Abigail M. Zellmer, Ian H. Guldner, Cody Kankel, Siyuan Zhang, Scott S. Howard, and Cody J. Smith, “Generating intravital super-resolution movies with conventional microscopy reveals actin dynamics that construct pioneer axons”, Development, Feb. 2019, doi: 10.1242/dev.171512. Supplementary software available at doi: 10.7274/r0-5hhg-5578.