{"id":318,"date":"2025-10-01T14:51:33","date_gmt":"2025-10-01T18:51:33","guid":{"rendered":"https:\/\/sites.nd.edu\/gtimp-lab\/?page_id=318"},"modified":"2026-02-05T09:39:26","modified_gmt":"2026-02-05T14:39:26","slug":"timp-nano-bio-lab-2","status":"publish","type":"page","link":"https:\/\/sites.nd.edu\/gtimp-lab\/","title":{"rendered":"G. TIMP NANO-BIO LAB"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>A LABORATORY AIMED AT MAKING A DIFFERENCE<\/strong>.   Through research, discovery, and innovation we shape the future of nanotechnology, biotechnology, health and medicine&#8230;<\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\"><strong>SYNTHETIC CAPILLARIES<\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-vimeo wp-block-embed-vimeo\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Composite-1\" src=\"https:\/\/player.vimeo.com\/video\/1117875108?dnt=1&amp;app_id=122963\" width=\"840\" height=\"287\" frameborder=\"0\" allow=\"autoplay; fullscreen; picture-in-picture; clipboard-write; encrypted-media; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\"><\/iframe>\n<\/div><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"341\" src=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure4021016a_2_original-paint-snip-7-1024x341.jpg\" alt=\"\" class=\"wp-image-945\" srcset=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure4021016a_2_original-paint-snip-7-1024x341.jpg 1024w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure4021016a_2_original-paint-snip-7-300x100.jpg 300w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure4021016a_2_original-paint-snip-7-768x256.jpg 768w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure4021016a_2_original-paint-snip-7.jpg 1145w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.nature.com\/articles\/srep21885\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>Above: Human erythrocytes (red blood cells) flowing in a synthetic capillary that was constructed using laser guided lithography<\/strong><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\">LASER GUIDED ASSEMBLY OF METAMATERIALS<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"782\" height=\"301\" data-id=\"752\" src=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Screenshotmodassembly-1.jpg\" alt=\"\" class=\"wp-image-752\" srcset=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Screenshotmodassembly-1.jpg 782w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Screenshotmodassembly-1-300x115.jpg 300w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Screenshotmodassembly-1-768x296.jpg 768w\" sizes=\"auto, (max-width: 782px) 85vw, 782px\" \/><\/figure>\n<\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202401344\" target=\"_blank\" rel=\"noreferrer noopener\">Above: <\/a><strong><a href=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adma.202401344\" target=\"_blank\" rel=\"noreferrer noopener\">Modular Assembly of 2D lattices of NPs using light gradients<\/a><\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\"><strong>PROTEIN SEQUENCING WITH A SOLID- STATE NANOPORE<\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"376\" src=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/fig-1-prot-seq-102425-85percent-1024x376.jpg\" alt=\"\" class=\"wp-image-1094\" srcset=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/fig-1-prot-seq-102425-85percent-1024x376.jpg 1024w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/fig-1-prot-seq-102425-85percent-300x110.jpg 300w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/fig-1-prot-seq-102425-85percent-768x282.jpg 768w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/fig-1-prot-seq-102425-85percent-1200x441.jpg 1200w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/fig-1-prot-seq-102425-85percent.jpg 1272w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"341\" src=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-1024x341.jpg\" alt=\"\" class=\"wp-image-1108\" srcset=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-1024x341.jpg 1024w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-300x100.jpg 300w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-768x255.jpg 768w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-1536x511.jpg 1536w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-2048x681.jpg 2048w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2080825c_protein-seq101625b-half-3-1200x399.jpg 1200w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdfdirect\/10.1002\/adfm.202515800\" target=\"_blank\" rel=\"noreferrer noopener\"><em>Peptide Sequencing<\/em><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em><strong>Above: <\/strong> (Top, left) A TEM image taken in situ of a pore immediately after sputtering through an a-Si membrane; <\/em>(center) <em> MD simulation of A<em>\ud835\udefd<\/em>1-42 transiting a sub-nanopore; <em> (right) Bi-conical topography of nanopore<\/em><\/em>. <strong> (<em>Bottom, <\/em><\/strong><em>left) A schematic cutaway of a single molecule biotin-A\ud835\udefd1\u221242 peptide, tethered to the tip of an AFM cantilever;  (center) Concomitant measurements of the force and current are shown as a single, biotin-A\ud835\udefd1\u221242 molecule was pulled once, through a sub-nanopore;(right) A kymograph of the AC current is shown that represents a compilation of auto-correlation functions.<\/em><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\"><strong>SINGLE CELL <\/strong>SECRETIONS USING A NANOPORE<\/h2>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"882\" height=\"432\" src=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/single-cell-secretions-5-resized.jpg\" alt=\"\" class=\"wp-image-1077\" srcset=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/single-cell-secretions-5-resized.jpg 882w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/single-cell-secretions-5-resized-300x147.jpg 300w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/single-cell-secretions-5-resized-768x376.jpg 768w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><figcaption class=\"wp-element-caption\">untitled<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.nanolett.8b01257\" target=\"_blank\" rel=\"noreferrer noopener\">Single Cell Secretomics <\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em><strong>Above<\/strong> (left) A schematic of the microfluidic cell conveyer and optical tweezers is shown; (b) A drawing of the seven-port microfluidic device with a nanopore embedded in the cross-bar is shown; (c) A false-color (perspective) reconstruction is shown of an MDA-MB-231 cell (green) suspended over a silicon nitride membrane with a nanopore in it.  Inset: A transmission electron micrograph (TEM) of a (2.8 nm \u00d7 2.9 nm \u2192) 6.4 nm<sup>2<\/sup> cross-section nanopore is shown; the shot noise highlighted by the dashed circle delineates the pore.<\/em><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center\"><strong>LIVE BACTERIAL PHYSIOLOGY WITH STEM<\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"335\" src=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-1024x335.jpg\" alt=\"\" class=\"wp-image-1015\" srcset=\"https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-1024x335.jpg 1024w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-300x98.jpg 300w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-768x251.jpg 768w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-1536x502.jpg 1536w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-2048x670.jpg 2048w, https:\/\/sites.nd.edu\/gtimp-lab\/files\/2025\/10\/Figure2092315a-live102125a-1200x392.jpg 1200w\" sizes=\"auto, (max-width: 709px) 85vw, (max-width: 909px) 67vw, (max-width: 1362px) 62vw, 840px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.5b07697?ref=article_openPDF\" target=\"_blank\" rel=\"noreferrer noopener\">STEM imaging of live bacterial physiology with a liquid cell<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><em>Above: <\/em><\/strong><em>(left) Image of negatively stained P1 phage on silicon nitride; (center) P1 phage adhering to K12 E. coli after staining; (right) The corresponding line scans that were used to evaluate the contrast and resolution .<\/em><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A LABORATORY AIMED AT MAKING A DIFFERENCE. Through research, discovery, and innovation we shape the future of nanotechnology, biotechnology, health and medicine&#8230; SYNTHETIC CAPILLARIES Above: Human erythrocytes (red blood cells) flowing in a synthetic capillary that was constructed using laser guided lithography LASER GUIDED ASSEMBLY OF METAMATERIALS Above: Modular Assembly of 2D lattices of NPs &hellip; <a href=\"https:\/\/sites.nd.edu\/gtimp-lab\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;G. TIMP NANO-BIO LAB&#8221;<\/span><\/a><\/p>\n","protected":false},"author":5046,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"footnotes":""},"class_list":["post-318","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>G. TIMP NANO-BIO LAB - TIMP NANO-BIO LAB<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.nd.edu\/gtimp-lab\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"G. TIMP NANO-BIO LAB - TIMP NANO-BIO LAB\" \/>\n<meta property=\"og:description\" content=\"A LABORATORY AIMED AT MAKING A DIFFERENCE. Through research, discovery, and innovation we shape the future of nanotechnology, biotechnology, health and medicine&#8230; SYNTHETIC CAPILLARIES Above: Human erythrocytes (red blood cells) flowing in a synthetic capillary that was constructed using laser guided lithography LASER GUIDED ASSEMBLY OF METAMATERIALS Above: Modular Assembly of 2D lattices of NPs &hellip; Continue reading &quot;G. 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