{"id":90,"date":"2022-05-13T19:56:31","date_gmt":"2022-05-13T23:56:31","guid":{"rendered":"https:\/\/sites.nd.edu\/nerenberg-lab\/?page_id=90"},"modified":"2023-08-21T09:28:02","modified_gmt":"2023-08-21T13:28:02","slug":"biofilms-and-human-health","status":"publish","type":"page","link":"https:\/\/sites.nd.edu\/nerenberg-lab\/research\/biofilms-and-human-health\/","title":{"rendered":"BIOFILMS AND HUMAN HEALTH"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\" style=\"font-size:28px;font-style:italic;font-weight:300\">Enhancing Antibiotic Efficiency with Low-frequency Ultrasound (LFU)<\/p>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\" style=\"background-color:#f2f4fa\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Combination therapy\" width=\"640\" height=\"360\" src=\"https:\/\/www.youtube.com\/embed\/l3DA7ACfJXA?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><figcaption class=\"wp-element-caption\">This figure shows how an antibiotic progressively inactivates Pseudomonas bacteria in a biofilm.  Green cells are live, and red cells are dead.  The deeper cells take much longer to inactivate.  <\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"wp-block-paragraph\">Low-frequency ultrasound (LFU), in conjunction with antibiotics, is known to produce a &#8220;bioacoustic effect,&#8221; increasing antiobiotic efficiency.  However, litttle is known about the bioacoustic effect for biofilms, which are more relevant to clinical infections. Understanding the efficiency of the bioacoustic effect for biofilms, and the underlying mechanisms, can lead to more efficient strategies for human infections, such as those resulting from cystic fibrosis.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The figure to the left shows how an antibiotic progressively inacticates Pseudomonas bacteria in a biofilm.  <\/p>\n<\/div>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:28px;font-style:italic;font-weight:300\">Impact of Biofilm Characteristics of the Efficiency of Low-frequency Ultrasound (LFU)<\/p>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\" style=\"background-color:#f2f4fa\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"457\" src=\"https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/08\/Bioacoustic-effect-1024x457.png\" alt=\"\" class=\"wp-image-760\" srcset=\"https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/08\/Bioacoustic-effect-1024x457.png 1024w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/08\/Bioacoustic-effect-300x134.png 300w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/08\/Bioacoustic-effect-768x343.png 768w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/08\/Bioacoustic-effect-1536x685.png 1536w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/08\/Bioacoustic-effect.png 1744w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">We are studying the effects of a range of biofilm characteristics, such as the biofilm thickness, density, and EPS composition, on the efficieny of the bioacoustic effect.  The figure to the left illustrates biofilm inactivation with and without the LFU. LFU greatly enhances inactivation.  <\/p>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:28px;font-style:italic;font-weight:300\">Effects of LFU on Microbial Resistance Mechanisms<\/p>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\" style=\"background-color:#f2f4fa\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-full is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"576\" height=\"576\" src=\"https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2022\/06\/Antibiotic-resistance.jpg\" alt=\"\" class=\"wp-image-488\" srcset=\"https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2022\/06\/Antibiotic-resistance.jpg 576w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2022\/06\/Antibiotic-resistance-300x300.jpg 300w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2022\/06\/Antibiotic-resistance-150x150.jpg 150w\" sizes=\"auto, (max-width: 576px) 100vw, 576px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"has-black-color has-text-color wp-block-paragraph\">LFU is believed to decrease antibiotic resistance. A possible mechanism might be related with ultrasound negatively affecting efflux pump activities, one of the main mechanisms of antibiotic resistance. <\/p>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:28px;font-style:italic;font-weight:300\">Low-frequency Ultrasound and Antibiotic Diffusivity<\/p>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\" style=\"background-color:#f2f4fa\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<figure class=\"wp-block-image size-large is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/02\/diffusivity-1024x576.jpg\" alt=\"\" class=\"wp-image-675\" srcset=\"https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/02\/diffusivity-1024x576.jpg 1024w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/02\/diffusivity-300x169.jpg 300w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/02\/diffusivity-768x432.jpg 768w, https:\/\/sites.nd.edu\/nerenberg-lab\/files\/2023\/02\/diffusivity.jpg 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p class=\"wp-block-paragraph\">One of the possible mechanism behind the bioacoustic effect is an increased in mass transfer. We are currently studying the effects of LFU in diffusion coefficients in several types of biofilms. <\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Enhancing Antibiotic Efficiency with Low-frequency Ultrasound (LFU) Low-frequency ultrasound (LFU), in conjunction with antibiotics, is known to produce a &#8220;bioacoustic effect,&#8221; increasing antiobiotic efficiency. However, litttle is known about the bioacoustic effect for biofilms, which are more relevant to clinical infections. Understanding the efficiency of the bioacoustic effect for biofilms, and the underlying mechanisms, can [&hellip;]<\/p>\n","protected":false},"author":4311,"featured_media":0,"parent":77,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-90","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/pages\/90","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/users\/4311"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/comments?post=90"}],"version-history":[{"count":15,"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/pages\/90\/revisions"}],"predecessor-version":[{"id":761,"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/pages\/90\/revisions\/761"}],"up":[{"embeddable":true,"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/pages\/77"}],"wp:attachment":[{"href":"https:\/\/sites.nd.edu\/nerenberg-lab\/wp-json\/wp\/v2\/media?parent=90"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}